Figure 4: Effect of Ac-PGP treatment on neovascularization during the cutaneous wound healing. | Scientific Reports

Figure 4: Effect of Ac-PGP treatment on neovascularization during the cutaneous wound healing.

From: N-Acetylated Proline-Glycine-Proline Accelerates Cutaneous Wound Healing and Neovascularization by Human Endothelial Progenitor Cells

Figure 4

(a) Representative H&E staining images of cutaneous wound in rats on day 6 after treatment of HBBS or Ac-PGP (0.1 μM) are shown. Functional blood vessels that include red blood cells are indicated by arrows. Bar = 100 μm. (b) Fluorescence images of ILB4 staining (green) in the rat dermal area at indicated days after introducing wound followed by treatment with HBSS or Ac-PGP (0.1 μM) are shown. Nuclei were counterstained with DAPI (blue). Bar = 100 μm. (c) Quantitative analysis of ILB4-positive blood vessels in the dermal area at indicated days after introducing wound is shown. Data indicate mean ± SD. *** < 0.001 versus HBSS (n = 8). (d) Fluorescence images of α-SMA staining (red) in the rat dermal area at indicated days after introducing wound followed by treatment with HBSS or Ac-PGP (0.1 μM) are shown. Nuclei were counterstained with DAPI (blue). Bar = 100 μm. (e) Quantitative analysis of α-SMA-positive vessels in the dermal area at indicated days after introducing wound is shown. Data indicate mean ± SD. *** < 0.001 versus HBSS (n = 8).

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