Figure 1: Norgestrel primes viable cells against microglial-derived toxicity.
From: Fractalkine-CX3CR1 signaling is critical for progesterone-mediated neuroprotection in the retina
(A) Quantification of TUNEL+ 661 W cells pre-treated with 20 μM Norgestrel or vehicle (DMSO) and in co-culture with C57 or rd10 microglia (N = 8 mice for primary culture, n = 6 technical replicates). (B) Example images of TUNEL+ 661 W cells (green) in co-culture with microglia. Scale bar 30 μm. (C) Quantification of TUNEL fluorescence intensity in P20 C57 explants treated with Norgestrel or vehicle and in co-culture with rd10 microglia (N = 3 explants, n = 4 technical replicates). (D) Example images of TUNEL reactivity (green) in the ONL of P20 C57 explants treated with Norgestrel or vehicle and in co-culture with rd10 microglia. Scale bar 50 μm. Hoechst reveals cell nuclei. Results are presented as mean ± SEM (t-test, **p < 0.01, ****p < 0.0001).
