Figure 2: Norgestrel-treated 661 W cells and C57 photoreceptors modulate microglial migration.
From: Fractalkine-CX3CR1 signaling is critical for progesterone-mediated neuroprotection in the retina
(A) Quantification of the number of 661 W cells pre-treated with 20 μM Norgestrel or vehicle (DMSO) contacted by rd10 microglia. (B) Quantification of the average number of microglia contacting 661 W cells in (A) (N = 8 mice for primary culture, n = 6 technical replicates). (C) Example images of 661 W cells (Cone Arrestin; red) pre-treated with Norgestrel or vehicle in co-culture with rd10 microglia. Scale bar 30 μm. (D) Example images of microglia (Iba1; red) and activated microglia (CD68; green) in untreated P20 C57 explants, and explants treated with Norgestrel or vehicle and in co-culture with rd10 microglia. Scale bar 50 μm. (E) Quantification of the number of microglia situated in the outer plexiform layer (OPL), outer nuclear layer (ONL) and outer segment layer (OSL) collectively, in untreated P20 C57 explants, and explants treated with Norgestrel or vehicle and in co-culture with rd10 microglia (N = 3 explants, n = 4 technical replicates). Hoechst reveals cell nuclei. Results are presented as mean ± SEM (t-test, *p < 0.05, **p < 0.01, ***p < 0.005).
