Figure 6: Soluble fractalkine is neuroprotective and modulates microglial migration in rd10 explants.
From: Fractalkine-CX3CR1 signaling is critical for progesterone-mediated neuroprotection in the retina
(A) Quantification of TUNEL fluorescence intensity in P15 rd10 explants treated with vehicle (0.1% BSA in 1 x PBS) or 100ng/ml recombinant soluble fractalkine (N = 4 explants, n = 4 technical replicates). Scale bar 30 μm. (B) Example images of TUNEL reactivity (green) in the ONL of rd10 explants treated with vehicle or soluble fractalkine. (C) Example images of microglia (Iba1; red) and activated microglia (CD68; green) in rd10 explants treated with vehicle or recombinant soluble fractalkine. Scale bar 50 μm. (E) Quantification of the number of microglia situated in the ONL in rd10 explants treated with vehicle or recombinant soluble fractalkine (N = 4 explants, n = 4 technical replicates). Hoechst reveals cell nuclei. Results are presented as mean ± SEM (t-test, ****p < 0.0001).
