Figure 2: PDN1 inhibits N1-Src activity in vitro. | Scientific Reports

Figure 2: PDN1 inhibits N1-Src activity in vitro.

From: Inhibition of N1-Src kinase by a specific SH3 peptide ligand reveals a role for N1-Src in neurite elongation by L1-CAM

Figure 2

GST-Y was incubated with 5 nM N1-Src and the indicated concentrations of either PDN1 or PDN1(P5A) and the extent of phosphorylation determined by densitometry of phosphotyrosine immunoreactivity. Representative immunoblots are shown in (a) and in (b) the data were plotted as arbitrary density units ± SEM, n = 3. (c) COS7 cells co-transfected with N1-Src-FLAG and CFP-PDN1 or CFP-PDN1(P5A) were lysed after 48 h and subject to immunoprecipitation with (IP) or without (−ab) anti-FLAG. The eluates were probed alongside 1% of the input lysate with anti-FLAG (top panels) anti-GFP (bottom panels).

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