Figure 5: Knockdown of N1-Src phenocopies the action of PDN1 in cultured neurons. | Scientific Reports

Figure 5: Knockdown of N1-Src phenocopies the action of PDN1 in cultured neurons.

From: Inhibition of N1-Src kinase by a specific SH3 peptide ligand reveals a role for N1-Src in neurite elongation by L1-CAM

Figure 5

(a) Schematic of the nucleotide and amino acid sequence of the N1-Src splice insert (red). Black bar indicates the sequence targeted by the N1-Src specific shRNA used in (b,c,d). (b) COS7 cells were transfected for 72 h with C-, N1- or N2-Src-FLAG and either pSUPER-GFP (control) or pSUPER-GFP encoding N1-Src specific shRNA. Cell lysates were probed with antibodies to FLAG or actin (loading control). (c,d) Cultured hippocampal neurons transfected with pSUPER-GFP (control) or pSUPER-GFP encoding N1-Src specific shRNA were analysed for length of longest neurite at 48 or 96 h. Scale bar = 20 μm. Data were normalised to the 48 h control and plotted as mean ± SEM, n = 90 neurons, with 30 cells analysed per condition in 3 independent experiments. Statistical analysis was performed by Kruskal-Wallis and post-hoc Dunn test (*P < 0.05; ***P < 0.001).

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