Figure 6: PDN1 inhibits neurite elongation mediated by L1-CAM. | Scientific Reports

Figure 6: PDN1 inhibits neurite elongation mediated by L1-CAM.

From: Inhibition of N1-Src kinase by a specific SH3 peptide ligand reveals a role for N1-Src in neurite elongation by L1-CAM

Figure 6

(a) Schematic of the poly-D-lysine (polyLys) coated coverslips treated with a droplet of L1-CAM and FITC. Inset shows the visible border between FITC/L1-CAM and polyLys coating. (b) Cultured cerebellar granule neurons transfected with CFP were grown on the indicated concentrations of L1-CAM and the length of the longest neurite (axon) determined. (c,d) CGNs were plated on polyLys coated coverslips with a region of 100 μg/ml L1-CAM (see a) and transfected after 24 h with CFP (control) or CFP-PDN1 for 48 h prior to fixing and processing for fluorescence microscopy. Length of longest neurite (c) and branches on the longest neurite (d) were quantified for neurons in the polyLys or L1-CAM regions using the NeuronJ plugin for ImageJ. All data were plotted as mean ± SEM, n = 3 experiments with 30 cells analysed per condition for each experiment. Statistical analysis was performed by one way ANOVA and post-hoc Tukey test (*P < 0.05; **P < 0.01; n.s = not significant).

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