Figure 5: Effects of LOS on AT1R, PKCγ, GTP-Rac1 (Pull-down Assay) and Cu/Zn-SOD proteins expression, and effects of LOS on AT1R, PKCγ and Cu/Zn-SOD mRNA level in the PVN of salt-induced hypertensive rats. | Scientific Reports

Figure 5: Effects of LOS on AT1R, PKCγ, GTP-Rac1 (Pull-down Assay) and Cu/Zn-SOD proteins expression, and effects of LOS on AT1R, PKCγ and Cu/Zn-SOD mRNA level in the PVN of salt-induced hypertensive rats.

From: Renin-angiotensin system acting on reactive oxygen species in paraventricular nucleus induces sympathetic activation via AT1R/PKCγ/Rac1 pathway in salt-induced hypertension

Figure 5

High salt (HS) intake rats had higher levels of AT1R, PKCγ and GTP-Rac1, but lower level of Cu/Zn-SOD compare with control rats. Bilateral PVN infusion of LOS for 14 days attenuated expression of AT1R, PKCγ and GTP-Rac1, and augmented expression of Cu/Zn-SOD in hypertensive rats. (A) A representative immunoblot; (B) PVN tissues were analyzed for Rac1 activity (Rac1-GTP) as described in METHODS. As controls, protein levels of Rac1 (total-Rac1) in PVN were measured. (C) Densitometric analysis of protein expression of AT1R, PKCγ and Cu/Zn-SOD in the PVN in different groups; (D) Densitometric analysis of mRNA expression of AT1R, PKCγ and Cu/Zn-SOD in the PVN in different groups. Values are expressed as means ± SE. *P < 0.05 vs NS groups (NS + PVN LOS or NS + aCSF); †P < 0.05 HS + LOS vs HS + aCSF.

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