Figure 1: Schematic overview of carbohydrate array construction and use. | Scientific Reports

Figure 1: Schematic overview of carbohydrate array construction and use.

From: Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger

Figure 1

After formation of a hydrophobic SAM of alkanethiols on a gold-coated target, hexadecylamine-labelled oligosaccharides are immobilised via hydrophobic-interaction. Products of enzyme activity, here exemplified by removal of DP2, as well as the original substrate, can be identified with MALDI-ToF MS.

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