Figure 4: MALDI-ToF MS analysis of commercial enzyme activity on carbohydrate arrays. | Scientific Reports

Figure 4: MALDI-ToF MS analysis of commercial enzyme activity on carbohydrate arrays.

From: Application of carbohydrate arrays coupled with mass spectrometry to detect activity of plant-polysaccharide degradative enzymes from the fungus Aspergillus niger

Figure 4

Spectra showing reaction products of incubation of (A) β-glucosidase on cellotetraose-containing arrays, with DP4 + Na 914.5 m/z, DP4 + H 892.5 m/z, DP3 + Na 752.4 m/z, DP3 + H 730.5 m/z, (B) endoxylanase on xylohexaose-containing arrays with DP6 + H 1036.7 m/z, DP5 + H 904.6 m/z, DP4 + H 772.6 m/z, DP3 + H 640.6 m/z. Reaction conditions were chosen such that incomplete substrate degradation allowed identification of both substrates and products in one mass spectrum. Intensities of peak resulting from background substrate degradation or impurities were ≤2.2% (Fig. S3).

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