Figure 1 | Scientific Reports

Figure 1

From: Highly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families

Figure 1

(A) Schematic representation of the use of IQF substrates in protease assays. (B) Structures of two donor molecules: ACC and MCA. Preliminary experiments allowed us to propose that ACC has a higher quantum yield; thus, substrates containing this donor moiety are more protease sensitive. ACC-Lys(DNP) is a new donor-acceptor pair for use in protease assays.

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