Figure 4: Rapid kinetics and quantitative assessment of prions from slice cultures using RT-QuIC assay. | Scientific Reports

Figure 4: Rapid kinetics and quantitative assessment of prions from slice cultures using RT-QuIC assay.

From: Integrated Organotypic Slice Cultures and RT-QuIC (OSCAR) Assay: Implications for Translational Discovery in Protein Misfolding Diseases

Figure 4

(A) The average SD50/g of protein (Spearman-Kärber estimates) of slice cultures was calculated based on endpoint dilutions from (n = 6 technical replicates) and plotted at time points (1, 7, 14, 21, 35 and 42 DPI) for both WT (n = 3 biological replicates) and Tga20 (n = 1 biological replicate) slice cultures. (B) The amyloid formation rate (AFR) was estimated by using the inverse of time in hours at which a trace crosses threshold fluorescence. The linear response AFR for cultured slices was both time- and seed concentration-dependent. (C) Dot-blot showing immunoreactivity against POM1 antibody. Progressive increase in immunoreactivity for PrP with respect to time spent in culture, starting at 14 dpi. Immunoreactivity against β-actin was used as a loading control for normalization. (D) Quantitative estimation of PrP immunoblot signal intensity.

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