Figure 5: OSCAR model to determine the anti-prion activity of compounds. | Scientific Reports

Figure 5: OSCAR model to determine the anti-prion activity of compounds.

From: Integrated Organotypic Slice Cultures and RT-QuIC (OSCAR) Assay: Implications for Translational Discovery in Protein Misfolding Diseases

Figure 5

(A–C) Compounds known to have anti-prion activity against RML scrapie prions were tested. RT-QuIC responses shown for 3 different seed concentrations (5 ng, 0.5 ng, and 50 pg) with or without compounds. Each trace shown is an average of 4 replicates (n = 2 biological replicates). (D,E) Quantification of anti-prion activity of compounds with respect to percent ThT-positive wells at different seed dilutions. Amyloid formation rate (AFR) represents the anti-prion activity of compounds tested.

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