Figure 2: Expression of r-bursicon proteins and bioassay.
From: Relish2 mediates bursicon homodimer-induced prophylactic immunity in the mosquito Aedes aegypti
(A) Western blot detection of r-bursicon proteins under reducing (left panel) and non-reducing (right panel) conditions using anti-His-tag antibody. Bursicon monomers formed dimers when expressed in vitro. (B) Neck-ligation delayed the cuticle tanning in mosquitoes. Photograph was taken at 30 min after emergence. (C) r-Bursicon heterodimer induced cuticle tanning in neck-ligated mosquitoes. Photograph was taken at 30 min after r-bursicon treatment. (D) Ae. aegypti r-busicon treatments led to D. melanogaster cuticle tanning. Photograph was taken at 30 min after r-bursicon treatment.
