Figure 4: TLR2 activation induces bioenergetics changes in RASFC in vitro. | Scientific Reports

Figure 4: TLR2 activation induces bioenergetics changes in RASFC in vitro.

From: Resolution of TLR2-induced inflammation through manipulation of metabolic pathways in Rheumatoid Arthritis

Figure 4

(a) (i) Representative cropped western blot demonstrating expression of PKM2 in RASFC whole cell lysates and nuclear fragments in response to Pam3CSK4 (1 μg/ml) stimulation (n = 3). (ii) Representative photomicrographs demonstrating Pam3CSK4-induced nuclear localisation of PKM2 as indicated by white arrows. Blue arrows represent lack of staining in nuclei under basal conditions. Mag 20X. (n = 3) (b) Representative oxidative phosphorylation (oxygen consumption rate (OCR)) and glycolysis (extracellular acidification rate (ECAR)) Seahorse bioenergetics profiles before and after injections of oligomycin, FCCP and antimycin A for RASFC in the presence and absence of Pam3CSK4 (1 μg/ml). Bar graphs demonstrating (c), baseline respiration (OCR), (d) the ratio of glycolysis: oxidative phosphorylation (ECAR:OCR), (e) ATP synthesis, (f) maximal respiration and, (g) the respiratory reserve in RASFC in response to Pam3CSK4 (1 μg/ml) stimulation (n = 7). Data was analysed using Wilcoxin Signed Rank test and is represented as Mean ± SEM, *p < 0.05, **p < 0.01 significantly different to control.

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