Figure 4: Differential expression and splicing of S. scrofa lncRNAs during skeletal muscle development. | Scientific Reports

Figure 4: Differential expression and splicing of S. scrofa lncRNAs during skeletal muscle development.

From: Comprehensive analysis of long non-coding RNAs highlights their spatio-temporal expression patterns and evolutional conservation in Sus scrofa

Figure 4

(A) Differential expression of S. scrofa lncRNAs among 3 developmental stages of skeletal muscle based on RNA sequencing data (Q value < 0.05). Muscle_0,_30 and_240 means skeletal muscle at day 0, day 30, and day 240 after birth. Each row represents data for one lncRNA. LncRNAs were clustered using hierarchical clustering. Gray indicates high expression level; light indicates low expression (see Supplementary Table S6 for the IDs of these differentially expressed lncRNAs). (B) Venn diagram representing the number and proportion of differentially expressed lncRNAs detected at each the developmental stage; D0, D30, and D240 indicate day 0, day 30 and day 240 after birth. (C) Validation of differential lncRNA expression by qPCR (red line with error bars). The RPKM value from deep sequencing data is shown for comparison (blue line). (D) Statistics of differential splicing events (S. scrofa lncRNAs and protein-coding genes) during muscle development based on RNA sequencing data, including mutually exclusive exons (MXEs), alternative 5′ splice sites (A5SSs), alternative 3′ splice sites (A3SSs), retained introns (RIs), and skipped exons (SEs). (E) Validation of differential splicing of a S. scrofa lncRNA by RT-PCR.

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