Figure 4: Snail1 reverses the effects of NA on E-cadherin expression.
From: Nicotinic acid inhibits glioma invasion by facilitating Snail1 degradation
U251 cells were treated with PBS (control) or 7.0 mM NA for 4 hr, or transfected with a plasmid expressing Snail1 and then treated with 7.0 mM NA for 4 hr. (A) Western blot analyses for whole-cell lysates were performed with an anti-E-cadherin antibody. Membranes were stripped and reblotted for Snail1 and β-actin. Representative images of Western blots are shown in A, and relative intensity of E-cadherin and Snail1 normalized against β-actin was calculated and summarized in (B,C) respectively. (D–F) Total RNA was extracted, and RT-PCR was carried out for the transcripts of E-cadherin, snail1, and GAPDH. Representative images of semi-quantitative RT-PCR are shown in (D), and relative expression levels of E-cadherin and snail1 (normalized against GAPDH), as determined by RT-qPCR, are shown in E and F, respectively. NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001. (G–L) Cells were fixed and processed for DAPI staining (blue) and immunocytochemistry for E-cadherin (green).
