Figure 1: Optimization of cryopreservation conditions for whole testes of medaka by vitrification.
From: Production of the medaka derived from vitrified whole testes by germ cell transplantation
(A,B) Viability of testicular cells after exposure to a hypertonic solution at 25 °C for 10 min (A) (n = 7) and at 0 °C for 30 min (B) (n = 9). Viability of testicular cells after exposure to each cryoprotectant solution at 25 °C for 30 min followed by a 0.2 M sucrose solution at 0 °C for 5 min (C) (n = 8). Volume changes in testicular cells following 30 min at 0 °C in each cryoprotectant solution (D) (n = 5–6). Testes on copper mesh (E). Survival of GFP-positive cells in testicular cells after cryopreservation with a vitrification solution (21% [w/v) ficoll and 0.35 M sucrose], including 30% (v/v) PG or 30% (v/v) EG in a plastic straw or on copper mesh (F) (n = 8). Testes and testicular cells (olvas-GFP) without cryopreservation (G,H) and after cryopreservation (I,J). Data are shown as the mean ± SEM (*P < 0.05; ***P < 0.001). Scale bars, 1 mm (E,G), and 10 μm (H).
