Figure 4: Production of an inbred line (Kaga) and endangered wild population (Tokyo medaka) derived from cryopreserved whole testes.
From: Production of the medaka derived from vitrified whole testes by germ cell transplantation
(A,B) Sperm and embryos derived from olvas-GFP male. (C,D) Sperm and embryos derived from olvas-GFP triploid recipients after transplantation of cryopreserved Kaga inbred line TCs. (E) PCR analysis, performed with tyrosinase-specific primers and gfp-specific primers, of F1 offspring derived from olvas-GFP surrogate triploid recipients by cryopreserved Kaga TC transplantation (lane F1), olvas-GFP medaka recipients (lane R1-2) and the donor Kaga inbred line (lane D1-2). (F) Medaka individuals (black-colored Kaga inbred line) generated from surrogate triploid olvas-GFP parents by cryopreserved TC transplantation. (G) PCR analysis, performed with tyrosinase-specific primers and gfp-specific primers and RFLP analysis for cytochrome b, of F1 offspring derived from olvas-GFP surrogate triploid recipients by cryopreserved Tokyo medaka TC transplantation (lane F1), donor Tokyo medaka (lane R1-R2), and olvas-GFP medaka recipients (lane D1-2). (H) Medaka individuals (black-colored wild population of Tokyo medaka) generated from surrogate triploid olvas-GFP parents by cryopreserved TC transplantation. Scale bars, 1 mm (B), and 10 μm (A).
