Figure 10: Identification of the size of PPARγ activating compound in the CMs. | Scientific Reports

Figure 10: Identification of the size of PPARγ activating compound in the CMs.

From: Commensal gut bacteria modulate phosphorylation-dependent PPARγ transcriptional activity in human intestinal epithelial cells

Figure 10

A. parvulum and P. copri CMs were fractions into fractions >100 kDa, >50 kDa, >30 kDa, >10 kDa, >3 kDa and >1 kDa. The lowest fraction showing activation on PPARγ reporter cell lines and the highest fraction showing loss of activity are represented for P. copri (A) and A. parvulum (B). The activating compound produced by P. copri is smaller than 3 kDa and bigger then 1 kDa. A. parvulum loses activity significantly after filtering using a 100 kDa filter. Experiments were performed in triplicates using two independent bacterial cultures and normalized to the activating CMs. Data were analyzed applying an ANOVA test followed by a post-hoc Tuckey test. Significance levels are indicated as follows: ***P < 0.001, **P < 0.005, *P < 0.05.

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