Figure 3: TRAPPC12 directly binds to the outer coat complex of COPII vesicle.
From: Mammalian TRAPPIII Complex positively modulates the recruitment of Sec13/31 onto COPII vesicles
GFP-TRAPPC12 or GFP-TRAPPC8 was overexpressed with indicated combinations of plasmids expressing Myc- or GFP-tagged COPII coat subunit(s) in 293T cells. Immunoprecipitations were performed using 9E10 anit-c-Myc antibody and detections of co-precipitated proteins were detected by immunoblottings using the indicated antibodies (anti-c-Myc or anti-GFP). Approximately 1% of the input lysates and 20% of the immunoprecipitates were loaded. (A) GFP-TRAPPC12 (GFP-C12) was tested for interaction with Myc-Sec13 alone or with GFP-Sec31A. (B) GFP-TRAPPC12 was tested for interaction with Myc-Sec23A alone or with GFP-Sec24C. (C) GFP-TRAPPC8 (GFP-C8) was tested for interaction with Myc-Sec13 alone or with GFP-Sec31A. (D) GFP-TRAPPC8 was tested for interaction with Myc-Sec23A alone or with GFP-Sec24C.
