Figure 4: Analysis of the early secretory pathway in TRAPPC12−/− cells.
From: Mammalian TRAPPIII Complex positively modulates the recruitment of Sec13/31 onto COPII vesicles
(A) Wildtype and TRAPPC12−/− HeLa cells were investigated with indicated markers of the early secretory pathway. (B) Wildtype and TRAPPC12−/− HeLa cells were stained with ERGIC53 after the cells were treated with or without autophagy inhibitor MHY 1485 at 10 μM for 2 hours. (C) Lysates of wildtype and TRAPPC12−/− HeLa cells were immunoblotted to detect the indicated proteins. (D) Wildtype and TRAPPC12−/− HeLa cells were depleted with siRNA specific to Firefly luciferase (control) or TRAPPC8 sequence and then stained with antibody against ERGIC-53. Scale bars = 10 μm.
