Figure 6: FRAP analysis of GFP-Sec31A wildtype and TRAPPC12−/− HeLa cells.
From: Mammalian TRAPPIII Complex positively modulates the recruitment of Sec13/31 onto COPII vesicles
(A) Wildtype (square) or TRAPPC12−/− (triangle) HeLa cells transiently transfected with GFP-Sec31A was bleached by laser and the recoveries of GFP fluorescence were recorded. Examples of detailed FRAP operations were documented in Supplementary Figure 5. (B) Wildtype (square) or TRAPPC12−/− (triangle) HeLa cells transiently transfected with GFP-Sec24C were bleached by laser and the recoveries of GFP fluorescence were recorded. n = 7; Error bars = S.D.
