Figure 3: Distribution of FP+ cells throughout the AF at P30, P40, P50.
From: A reliable and flexible gene manipulation strategy in posthatch zebra finch brain
(a) Schematic of AF and its subregions, CMM, NCM, and Field L. Dashed lines indicate the four rostral-caudal planes spanning the dorsal-ventral axis of AF used for Plot profile analysis: Dorsal, Central, Ventral, and Super Ventral. (b) Representative greyscale images of the AF in P30, P40, and P50 males and females. Contrast was adjusted for figure production only. Scale bar = 200 μm. (c) Plot profile analysis, quantifying the mean grey values along the four lines depicted in (b). Sections 165 μm (left column), 495 μm (middle column), and 825 μm (right column) from midline were analyzed. Central, Ventral, and Super Ventral lines included Field L, denoted with grey bars. Graphs are centered around Field L for visual comparison. Note that x-axes vary along the dorsal-ventral axis, reflecting the change in the anatomical length of AF. Lines represent the age group mean. n = 4 (P30), and 3 (P40 and P50).
