Figure 4: Colocalization of fluorescent proteins and NeuN.
From: A reliable and flexible gene manipulation strategy in posthatch zebra finch brain
(a) Schematic of the areas imaged for the dCMM, dNCM, vCMM, and vCMM quantifications. (b) Example image of the visual confirmation of FP+ cells that are also NeuN-positive (NeuN+; arrowhead), and FP+ cells that are NeuN-negative (arrow). Scale bars = 25 μm. (c and e) Proportion of FP+ cells that are also NeuN+. *p < 0.01, as compared with the P30 and P50 age groups within vNCM. (d and f) Proportion of NeuN+ cells that were also FP+. *p < 0.01, as compared with the area-matched P30 age group. Data are presented as the proportion of co-labeled cells within age and across brain area (c and d) or within brain area and across age (e and f). Bar graphs represent the group mean ± SEM; ○ = individual birds. n = 4 (P30), and 3 (P40 and P50).
