Figure 5: Synapsin promoter enhances neuronal expression of fluorescent proteins.
From: A reliable and flexible gene manipulation strategy in posthatch zebra finch brain
The hSyn1 promoter was used to drive eGFP expression and CAG was used to drive universal transcription of RFP. (a) Density of either RFP-positive (RFP+) or eGFP-positive (GFP+) in AF (left graph), CMM (middle graph), or NCM (right graph) at P30 and P40. *p < 0.05 and **p < 0.001, as compared with age-matched RFP+. (b) Proportion of RFP+ or GFP+ cells that are also NeuN+. Data are presented as the mean across the dCMM, vCMM, dNCM, and vNCM subregions of AF. *p < 0.05 between indicated groups. (c) Proportion of NeuN+ cells that are also either RFP+ or GFP+ in each AF subregion at P30 (left graph) and P40 (right graph). *p < 0.05 between indicated groups. All data are presented as the group mean ± SEM; ○=individual birds. n = 3 per age.
