Figure 1: BC16 construct.
From: Limitations and challenges of genetic barcode quantification
(a) BC16 barcodes were introduced in front of the 3′ LTR of the LeGO vector. They consist of 16 random nucleotides separated by fixed triplets. (b) Results of the quantitative digital droplet PCR (ddPCR) which was performed directly after generating the miniBulks. (c) Overview of the minimal HD of the picked barcodes. (d) The frequency plot of one particular miniBulk depicts all identified barcodes (on a log scale) with more than two reads in a descending order. Every barcode is coloured according to its minimal HD to one of the original barcodes. The frequencies of the original five barcodes are depicted in the inset. (e) Sequence similarities visualized with a network based graph (ripple plot). Barcodes are represented as nodes, sequence similarities of HD = 1 between two barcodes are visualized as links and node sizes reflect read counts. The minimal HD of each barcode to one of the original barcodes is again color-coded according to the depicted legend. (f) The average abundance of all original barcodes over all replicates after 65 cycles of PCR.
