Figure 2: Influence of different PCR-cycle quantities – BC16.
From: Limitations and challenges of genetic barcode quantification
Influence of a decreasing number of PCR cycles on the generation of descendent barcodes visualized as a network based graph (ripple plot). Barcodes are represented as nodes, sequence similarities with a HD = 1 are visualized as links and node sizes reflect read counts. The minimal nucleotide differences between each barcode and one of the original barcodes is color-coded as shown in the legend. Original barcodes are “red” (HD = 0), descended barcodes with a HD = 1 are “orange” and so on as depicted in the color scale. Shown is a PCR cycle reduction without restriction enzyme treatment.
