Figure 3: PCR protocol modifications – BC16.
From: Limitations and challenges of genetic barcode quantification
Every barcode mixture was analysed using a successive reduction of PCR cycle numbers from 65 down to 30 PCR cycles in combination with different sample preparation strategies, namely the (a) standard protocol (gDNA), (b) an improved protocol with an additional restriction digest (EcoRI) and (c) a totally artificial protocol consisting of previously build and afterwards mixed 1 kb long DNA fragments (1 kb). The relative average barcode abundances of all miniBulk replicates are shown as barplots.
