Figure 5: PCR protocol modifications – BC32.
From: Limitations and challenges of genetic barcode quantification
Every barcode mixture was analysed using a successive reduction of PCR cycle numbers from 40 down to 20 PCR cycles in combination with different sample preparation strategies, namely the standard protocol (gDNA), an improved protocol with an additional restriction digest (EcoRI) and a totally artificial protocol consisting of previously build and afterwards mixed 1 kb long DNA fragments (1 kb). Depicted are the average barcode abundances including their standard deviation of all miniBulk replicates.
