Figure 6: Spike-in experiments – BC32.
From: Limitations and challenges of genetic barcode quantification
In a dilution series, the four BC32 cell clones are mixed into non-transduced (untx) and also barcode-transduced HEK293T cells (Cer-BC32) to obtain mixtures containing 10%, 1%, 0.1% and 0.01% of each clone. The average barcode abundances in the replicates are visualized as barplots including their standard deviation. Only the relative read counts of the four original barcodes are analysed.
