Figure 4: Autophagic flux is impaired in acute and chronic DOX-induced cardiotoxicity.

(a) Western blot detection of LC3 II and p62 protein abundance in LVs from mice 5 days after acute DOX treatment and further elicited by CQ treatment. (b) Quantification of LC3-II protein abundance as illustrated in panel (a). n = 3 mice for each group. (c) Quantification of p62 protein abundance as illustrated in panel (a). n = 3 mice for each group. (d) Western blot detection of LC3 II and p62 protein abundance in LVs from mice treated with DOX over 4 weeks and further elicited by CQ treatment. (e) Quantification of LC3-II as illustrated in panel (d). n = 3 mice for each group. (f) Quantification of p62 as illustrated in panel (d). n = 3 mice for each group. *P < 0.05 vs. vehicle. ^#P < 0.05 vs. CQ.