Figure 6: Ranavirus phylogeography of the Iberian Peninsula.

Portuguese newt and toad ranavirus (PNTRV) relationships to other known Iberian ranaviruses of wild herpetofauna. Year of first observed outbreaks of CMTV-like ranaviruses (green), FV3-like ranaviruses (red) and an undetermined lineage (yellow). PNTRV infecting amphibians is embedded in the CMTV-like clade, while LMRV has been found in Serra da Estrela population of Iberolacerta monticola and is part of the FV3-like group. Phylogeny constructed from concatenated alignments of six partial genes (see main text). The final concatenated alignment was 2015 bp in length. Node support values were annotated on the best maximum-likelihood tree and were calculated using maximum likelihood (100 bootstraps, black) and Bayesian inference (posterior probabilities as percentage, red). Scale of branch lengths is in nucleotide substitutions per site. Abbreviations key: FGS, Tanque de Folgosinho; LCS, Lagoa dos Cântaros; RTR, Represa da Torre; LCQ, Lagoa do Covão das Quelhas. Points on the map were generated using QGis 2.0 (Quantum GIS Development Team, 2013) and edited on Adobe PhotoShop CS6 (Adobe, 2012).