Figure 2: Characterization of uterine phenotype. | Scientific Reports

Figure 2: Characterization of uterine phenotype.

From: High-Fat Diet Induces Unexpected Fatal Uterine Infections in Mice with aP2-Cre-mediated Deletion of Estrogen Receptor Alpha

Figure 2

(A,B) Representative macro- and microscopic images of uteri of atERαKO vs. wt-mice fed a CD or HFD. (C) Quantification of the grade of inflammation (n = 9–17). Legend: 0 = no inflammation; 1 = beginning inflammation; 2 = visible inflammation; 3 = severe inflammation (D) Representative Mac3- and Ly6G-stainings. (E) Quantification of macrophages (Mac3) and neutrophils (Ly6G) in uteri of atERαKO mice (n = 9–17). Legend: 0 = no immune cells; 1 = few immune cells; 2 = high quantity of immune cells; 3 = very high quantity of immune cells (F) Relative mRNA-expression of CD47 and PAI-1 normalized to Ly6G mRNA-expression in uteri, comparing atERαKO mice on CD or HFD (n = 4–7). Relative Nos2 and Arg1 mRNA expression in uteri (G) and white adipose tissue (H) of atERαKO mice (n = 7–8). *P < 0.05, **P < 0.01 two-tailed t-test or 2-way-ANOVA (Bonferroni-posttest).

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