Figure 4: Impact of stearic acid on macrophage polarization (and function). | Scientific Reports

Figure 4: Impact of stearic acid on macrophage polarization (and function).

From: High-Fat Diet Induces Unexpected Fatal Uterine Infections in Mice with aP2-Cre-mediated Deletion of Estrogen Receptor Alpha

Figure 4

Characterization of M1- and M2-markers on differentiated THP-1 cells after stimulation with estradiol (E2)+BSA, E2+C18:0, E2+LPS and E2+LPS+C18:0. (A) Relative mRNA-expression of CCR7 (M1) and CD206 (M2) over E2+LPS stimulated cells. (B) Representative images of CD209 (M2-marker) staining of E2+BSA or E2+C18:0 stimulated THP-1 cells. (C) Representative FACS-dot plots of CD11b and CD209-staining. First column represents unstained cells in forward (FSC) and side scattered blots (SSC); second column shows staining of CD11b- (y-axis) and 7AAD- (x-axis) staining. The third column is analogous to the second one for CD209-staining. Average MFI of viable (7AAD negative) CD11b positive cells (left upper quarter of the blots) and MFI of viable CD209 cells are indicated. (D) Relative mean-fluorescence-intensity of cd11-positive cells and (E) relative mean-fluorescence-intensity of cd209-positive cells (x-fold induction over E2/LPS-stimulated cells). (F) Representative images of phagocytotic analysis of E2+BSA or E2+C18:0 stimulated THP-1 cells. **P < 0.01, ***P < 0.001 vs. E2+BSA; #P < 0.05, ###P < 0.001 vs. E2+LPS (n = 3; one-way-ANOVA).

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