Figure 4: ACCORD results for parallel coiled-coils (CCs).
From: ACCORD: an assessment tool to determine the orientation of homodimeric coiled-coils
Size-exclusion chromatography with multi-angle light scattering data from (a) SspB-GCN4, (b) SspB-APC, (c) SspB-Atg16, (d) SspB-SCOC, (e) SspB-LRRFIP1, (f) SspB-Ndel1, (g) SspB-TPM1, and (h) SspB-ROCK1 fusion proteins. Insets show the results of sodium dodecyl sulphate-polyacrylamide gel electrophoresis of the chromatography fractions. (i) Schematic model of the SspB-ROCK1 dimer. All parallel CCDs fused with the SspB protein behaved as dimers in solution.
