Figure 6: Validation of ACCORD results.
From: ACCORD: an assessment tool to determine the orientation of homodimeric coiled-coils
(a) Size-exclusion chromatography with multi-angle light scattering (SEC-MALS) data showed that SspB-NDP52 is a dimer in solution, suggesting that the coiled-coil domain (CCD) of NDP52 is oriented in a parallel manner. Inset shows the results of SDS-PAGE of the chromatography fractions. (b) Ab initio envelope using SAXS data superimposed onto two MBP molecules (PDB ID: 3DM0) and a parallel CCD of Atg16 (PDB ID: 3A7O), which might have similar CC lengths based on the numbers of residues. (c) Molecular envelope of MBP-MDV1. The high-resolution crystal structure of MBP-MDV1 (Supplementary Fig. 2) was fitted into the low resolution molecular envelope generated from SAXS data.
