Figure 1: P. aeruginosa formed biofilm-like structures on 3-D lung epithelial cells. | Scientific Reports

Figure 1: P. aeruginosa formed biofilm-like structures on 3-D lung epithelial cells.

From: Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum

Figure 1

(A) 3-D lung epithelial cells co-cultured without (a–c) or with (d–f) GFP-expressing P. aeruginosa PAO1 for 2 h, 6 h and/or 17 h. Immunofluorescence and light microscopy images at a magnification of 400x are overlaid. (B) Scanning electron microscopy images of P. aeruginosa biofilms (white dotted frame) at the 6 h infection time point with a magnification of 500x (left image) and 10,000x (right image) (C) Number of colony forming units (CFUs) adhering to the 3-D lung epithelial cells at different time points. (D) Number of CFUs present in the surrounding medium (not associated to host cells).

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