Figure 2 | Scientific Reports

Figure 2

From: Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum

Figure 2

Effect of P. aeruginosa biofilms on host cell viability and tight junction formation in 3-D lung epithelial cells, following infection for 2 h, 6 h, and 17 h (as compared to uninfected controls): (A) Trypan blue exclusion assay. (B) LDH release assay. LDH release is presented as a percentage of a positive control (3-D lung epithelial cells lysed with Triton-X100). (C) Immunofluorescence imaging of the tight junctional marker β-catenin (green) for uninfected controls (a–c) and infected samples (d–f), at a magnification of 400x. NS = non-significant (p > 0.05).

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