Figure 2: Effect of GTP hydrolysis on filament structure.
From: TubZ filament assembly dynamics requires the flexible C-terminal tail
(a) Critical concentration measurements of CbTubZ in buffer containing Ficoll (200 g/L) and GTP/Mg2+ or GDP/Mg2+ (b). Negative stain EM showing CbTubZ filaments polymerized in the presence of GDP/Mg2+. Inset shows averaged filaments and the corresponding Fourier transform. Arrow indicates 4.8-nm longitudinal spacing between molecules. (c) AFM images of CbTubZ filaments polymerized in the presence of GDP/Mg2+. The black line corresponds to the height profile shown in the graph. Color scale from dark to bright correspond to 0–11 nm. (d) Negative stain EM of CbTubZ filaments assembled with GTP/Mg2+ and sonicated for 10 min, showing the stiffening effect.
