Figure 4: TubY - TubZ interaction. | Scientific Reports

Figure 4: TubY - TubZ interaction.

From: TubZ filament assembly dynamics requires the flexible C-terminal tail

Figure 4

(a) Negative stain EM (left) and AFM (right) of CbTubZ-TubY filaments. The height profile shown in the graph was taken in the direction of the black arrow. (b) AFM images of CbTubZE200A-TubY filaments. White arrows indicate thinner extensions at the ends of some filaments. Color scale from dark to bright is 0–11 nm in AFM images. (ce) EM images of TubY binding to GDP-bound CbTubZ filaments (c), TubZ350 filaments and rings formed in the presence of TubY (d) and detail of ring formation upon disassembly from filaments tips (e, bottom). (e) Different types of averaged structures obtained after ring processing, including the number of monomers in each ring (top).

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