Figure 2: Generation of Rtfc knockout mice.
From: Rtfc (4931414P19Rik) Regulates in vitro Thyroid Differentiation and in vivo Thyroid Function
(a) Genotyping of the founder mice. Only one allele of Rtfc was modified in founder mice 1, 3, 5, 6, 7, 9 and 10, while both Rtfc alleles were modified in founder mouse 8. It appears that there were three Rtfc alleles in founder mouse 4. Neither Rtfc allele was modified in founder mouse 2. (b) Sanger sequencing result of the representative homozygous knockout mouse reveals a 1-bp deletion in two Rtfc gene alleles. The red box marks the deleted G in the Rtfc−/− (Δ1) mouse.
