Figure 4: ApoA-I of RRMS patients is modified at its tyrosine and tryptophan residues. | Scientific Reports

Figure 4: ApoA-I of RRMS patients is modified at its tyrosine and tryptophan residues.

From: Relapsing-remitting multiple sclerosis patients display an altered lipoprotein profile with dysfunctional HDL

Figure 4

(a–d) Collision-induced dissociation (CID) spectra of Trp-50 nitration (a), and Trp-50 mono-, di-, and tri-oxidation (b,c and d respectively). Spectra were acquired on analysis of in-gel tryptic digests of the ApoA-I band from HDL3 isolated with sequential flotation ultracentrifugation. Modifications were detected in an LC-MS/MS experiment as described under Methods. CID fragment ion annotation was according to the nomenclature by Roepstorff and colleagues108. (e) Summary of ApoA-I modification sites. Residues that were modified are indicated with ∆. The numbering of the amino acids cited refers to the amino acid sequence of the mature protein.

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