Figure 4: IL-27 enhances phosphorylation of p47^phox in macrophages.

IL-27 treated and untreated macrophages were stimulated with 100 ng/ml PMA for 20 min at 37 °C and then whole cell lysates were prepared in the presence of a phosphatase inhibitor and proteinase inhibitor. Western blot was performed with anti-phosphorylated p47^phox, anti-p47^phox, anti-gp91^phox, and anti-β-actin was used for detecting a loading control.