Figure 6: Analysis of the LAMP products with the LFD and portable sealed biosensor. | Scientific Reports

Figure 6: Analysis of the LAMP products with the LFD and portable sealed biosensor.

From: A smart sealed nucleic acid biosensor based on endogenous reference gene detection to screen and identify mammals on site

Figure 6

(A) Specificity test of the LFD-LAMP assay with LFD. Lane 1, pork; 2, beef; 3, sheep; 4, horse; 5, donkey; 6, mouse; 7, dog; 8, rabbit; 9, water buffalo; 10, camel; 11, sable; 12, deer; 13, yak; 14, monkey; 15, human placenta; 16, chicken; 17, duck; 18, goose; 19, fish; 20, trionyx; 21, bullfrog; 22, sparrow; 23, negative control. (B) Sensitivity test of the LFD-LAMP products by LFD. Lane 1, 100 ng; 2, 10 ng; 3, 1 ng; 4, 0.1 ng; 5, 10 pg; 6, 1 pg; 7, negative control. (C) LAMP positive and negative reactions were subjected to portable sealed biosensor visual detection. Lane 1, positive control; 2, negative control. All pictures were collected after 3 min.

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