Figure 1: Intrarectal inoculation of predatory bacteria into rats.
From: Effect of predatory bacteria on the gut bacterial microbiota in rats
Predatory bacteria were introduced by intracolonic instillation of Sprague Dawley rats. Animals were anaesthetized with 4% isoflurane in oxygen for five minutes. A 3Fr polyurethane catheter with a 1 mL syringe attached via a Luer Lock was inserted intrarectally to approximately 8 cm proximal to the anus, at which 250 μl of bacterial suspension (diluted in PBS) was slowly injected into the colon. In this image, Crystal Violet was inoculated to visualize where the inoculums would travel within the colon.
