Figure 5: Validation of lncRNA expression in microarray data by real time qRT-PCR.
From: Profiling analysis of long non-coding RNAs in early postnatal mouse hearts
(a) The expression levels of 5 consistently upregulated lncRNAs (left panel) and 5 consistently downregulated lncRNAs (right panel) during neonatal mouse heart development were validated by real time qRT-PCR. (b) The expression levels of the above-validated lncRNAs in different mouse tissues that contain smooth muscle cells were determined by real time qRT-PCR. Data are normalized to Gapdh. Data are shown as mean ± S.D. *, ** and ***indicate p < 0.05, p < 0.01 and p < 0.001, respectively, which denote statistical comparison between the two marked groups. One-way ANOVA for (a) (post hoc: LSD multiple comparison test for AK047066, uc008kgz.1, uc008grn.1, ENSMUST00000140716, uc009kob.1, ENSMUST00000136359, ENSMUST00000117266 and ENSMUST00000149974; Dunnett T3 multiple comparison test for ENSMUST00000153057 and AK009349) and (b) (post hoc: LSD multiple comparison test). (n = 3 samples per time point).
