Figure 1: Overexpression of ATL1 impaired neurite outgrowth in PC-12 cells.
From: Atlastin regulates store-operated calcium entry for nerve growth factor-induced neurite outgrowth
(a and b) Representative images for neurite outgrowth in GFP-transfected PC-12 cells with (b) or without (a) 100 ng/ml NGF treatment for 48 hours. (c–h) Images of NGF-treated PC-12 cells transfected with Sec61β-GFP (c) or co-transfected with GFP and Myc-ATL1-wt (d) Myc-ATL1-K80A (e) Myc-ATL1-Y196C (f) Myc-ATL1-R217Q (g) or Myc-ATL1-P342S (h). (i) Western blot for Myc-vector, Myc-ATL1-wt, Myc-ATL1-K80A, Myc-ATL1-Y196C, Myc-ATL1-R217Q, and Myc-ATL1-P342S in PC-12 cells. GAPDH was used as a loading control. Full length blot are presented in Supplementary Figure S6a. (j) Quantification of the cells with neurites longer than 15 μm. The percentage was determined from three independent assays. (Vector without NGF, n = 62; vector, n = 230; Sec61β-GFP, n = 134; ATL1-wt, n = 134; ATL1-K80A, n = 170; ATL1-Y196C, n = 112; ATL1-217Q, n = 141; ATL1-P342S, n = 165). Scale bar = 20 μm. *P < 0.05; **P < 0.01.
