Figure 3: Depletion of ATLs reduced SOCE.
From: Atlastin regulates store-operated calcium entry for nerve growth factor-induced neurite outgrowth
(a) Representative intracellular free calcium ([Ca2+]i) recordings showing TG-triggered SOCE in COS-7 cells transfected with control non-targeting siRNA ( left ) or siRNAs target ATL2 and ATL3 ( right ) for 72 h. (b) Averaged peak values of [Ca2+]i were collected from corresponding COS-7 cells (siCtrl, n = 70; siATL2/3, n = 71). (c) ATL2 and ATL3 expression was detected by immunoblotting. An asterisk (*) indicates a non-specific band. Full length blot are presented in Supplementary Figure S6c. (d) The ER morphology was visualized by calreticulin antibody staining. Enlarged areas are marked by the small squares, showing normal ( left ) and long, unbranched ER tubular networks ( right ). Scale bar = 15 μm. ***P < 0.001.
