Figure 5: Extracellular Ca2+ was essential for NGF-induced neurite outgrowth.
From: Atlastin regulates store-operated calcium entry for nerve growth factor-induced neurite outgrowth

(a) Representative images for neurite outgrowth in PC-12 cells treated with 2 mM Ca2+, NGF + 2 mM Ca2+, or NGF only and cultured in calcium-deficient DMEM (cdDMEM). (b) The percentage of neurited cells with neurites longer than 15 μm (No NGF, n = 342, NGF + Ca2+, n = 374; NGF only, n = 587). *P < 0.05.